Wigan Athletic, Blackburn Rovers and Wolverhampton Wanderers were among the Championship sides to exit the League Cup on a night of first-round shocks.
Defence was at a premium on an eventful night that saw Brentford beat Dagenham and Redbridge 4-2 on penalties after a staggering 12 goals were shared at 6-6 after extra-time.
But cup specialist Wigan, which won the FA Cup in 2013 before reaching the semi-finals in 2014, was the biggest side to exit the competition at the first stage, losing 2-1 at home to Burton Albion.
Midfielder Neal Bishop's 34th-minute strike condemned Blackburn Rovers to a 1-0 home loss to Scunthorpe United, while Northampton Town earned a thrilling 3-2 success at Wolverhampton Wanderers.
But each of those results were dwarfed by events at Victoria Road, where Dagenham and Redbridge suffered spot-kick agony after an astounding encounter against Brentford despite Ashley Hemmings's double.
Leyton Orient also went through 6-5 on penalties after a 3-3 draw with a spirited Plymouth Argyle side.
Ipswich Town was another second-tier side to exit as Izale McLeod's 111th-minute effort sealed a 1-0 win for Crawley Town, and beleaguered Blackpool was defeated 1-0 by Shrewsbury Town.
Managerless Huddersfield Town staged a dramatic comeback to beat Chesterfield 5-3 in its first game since the departure of Mark Robins.
Trailing 3-1 with six minutes remaining, the visitor scored two late goals through Nahki Wells and Jon Stead before the former completed his hat-trick to help cap the turnaround in extra-time.
Birmingham City needed extra-time to beat Cambridge United 3-1, while Bolton Wanderers came from behind to beat Bury 3-2 in 120 minutes.
Rotherham United required the extra 30 minutes to beat Fleetwood Town 1-0 but the majority of Championship teams all progressed with no such difficulties.
Middlesbrough, Sheffield Wednesday, Nottingham Forest, Brighton and Hove Albion, Bournemouth, Watford and Reading all enjoyed relatively straightforward routes into the next round.
Leeds United was a 2-1 winner over Accrington Stanley and Millwall knocked out Wycombe Wanderers 1-0 in outings that each saw the Championship side reduced to 10 men.
Ben Williamson scored a hat-trick as Hartlepool United was hammered 6-2 by Port Vale, whose League One counterparts Preston North End, Crewe Alexandra, Doncaster Rovers, Swindon Town, Charlton Athletic, Gillingham, Walsall and Milton Keynes Dons all emerged victorious.
Bristol City and Peterborough United each suffered surprise losses to Oxford United and Portsmouth respectively, while 2013 finalist Bradford City won 1-0 at Morecambe thanks to Aaron McLean's late goal.