Sevilla beat Roma 2-0 in the round of 16 on to stay in the hunt for a record sixth UEFA Europa League title, as Wolverhampton, Bayer Leverkusen and Basel also qualified for the quarter-finals.
Full-back Sergio Reguilon gave Sevilla the lead with a surging run through the Roma defence and a shot past keeper Pau Lopez in the 22nd minute.
Youssef En-Nesyri added the second just before halftime, tapping in a low cross from Lucas Ocampos.
Sevilla could easily have scored more, with two shots hitting the crossbar and a goal overturned for offside on video review.
Playing after a packed run of Serie A games, Roma didn't muster a single shot on target in the first half and just one in the second.
"It was a great performance in all senses. We will enjoy it tonight, recharge our batteries and be ready for our next final in a few days' time," Reguilon said.
It was a single game at a neutral venue in Germany because no first leg was played in March before the season was suspended amid the coronavirus pandemic.
No fans are allowed at any Europa League games.
It was Roma's first game since its $US700 million ($A968 million) takeover by the Texas-based Friedkin Group was confirmed on Thursday morning.
Sevilla next faces a quarter-final on Tuesday against Wolves after the English team squeezed past Olympiacos 1-0 on the night and 2-1 on aggregate for its first European quarter-final in 48 years.
Raul Jimenez scored the only goal from the penalty spot in the ninth minute after Olympiacos goalkeeper Bobby Allain gave away a spot-kick with a clumsy challenge after he failed to control the ball.
Both teams had plenty of chances to score in an action-packed second half but a combination of poor finishing and solid goalkeeping kept the score down.
Bayer Leverkusen earned a quarter-final against Inter Milan, beating Rangers 1-0 thanks to a powerful shot from Moussa Diaby for a 4-1 aggregate win.
Basel already had a 3-0 lead from the first leg and made the quarter-finals after Fabian Frei scored the only goal of the game in a 1-0 win over Eintracht Frankfurt.
Basel next plays Shakhtar Donetsk.
The teams in the final eight come from seven different countries, with only England having two - Wolves and Manchester United.