Racing Santander failed to make it past the first round of the Copa del Rey, losing 2-0 away at CD Laredo.
Racing, now in the Segunda B and managed by former Spain international Pedro Munitis, conceded two own goals in the first half at Campo San Lorenzo to fall to its fourth-tier opponent.
Elsewhere in round one, Tudelano defeated the 10 men of Compostela 3-2, while CD Varea also had a man sent off as it went down by the same scoreline at home to Leonesa.
Mensajero and CF Talavera de la Reina progressed thanks to 2-0 victories, but one goal was enough to send Guijuelo through at the expense of Pontevedra.
Arandina CF scored three goals in extra-time to see off Condal CF 4-1. Ebro, however, required penalties to knock out Real Union at home, the tie having finished level at 2-2 after 120 minutes.
There was also a shootout at Sabadell, with Castellon holding its nerve to knock out its host by a 4-2 scoreline.
SD Formentera made it through to the next round too, upsetting Alcoyano 2-1, while Reus, Linares Deportivo and Linense extended their cup runs for a little while longer.
Lleida Esportiu proved too strong for Hercules, triumphing 3-1, while a goal from Hugo Rodriguez saw Cadiz squeeze past Murcia.
Socuellamos saw off Portugalete 2-1 and, in the final tie of the night, Javi Medina found the net with eight minutes remaining to give Algeciras a 1-0 triumph away at Melilla.