Melbourne City will have to either play their A-League home semi-final against Macarthur FC behind closed doors at AAMI Park or have the game relocated after fans were banned from attending games in Melbourne.
City had held out hope of a small crowd being able to attend Sunday's game but those plans were scuppered by the Victorian government ruling out crowds in Melbourne this weekend, despite easing coronavirus restrictions.
The A-League delayed ticket sales on Thursday morning, with City later confirming fans would not be able to attend.
The game will now either be held behind closed doors in Melbourne, or relocated.
"We've just received word from the DHHS that we are unable to have crowds at Sunday's (semi-final)," City said on Twitter on Thursday.
"We pushed as hard as we could and are extremely disappointed by the decision.
"We're in discussions with the APL (Australian Professional Leagues) as to what will now happen to our fixture and will update you ASAP."
City earned a home semi-final by finishing top of the table, and will have the right to host the grand final if they progress to the decider.
On Wednesday, captain Scott Jamieson said he would prefer to play the game behind closed doors.
"Obviously I understand there's a commercial side of this business but at the end of the day, (if) you finish first, you should get the opportunity to play your home semi-final at home," he told SEN.