Defending champion Leon catapulted itself into play-off contention, while Toluca rose to second in the standings.
Striker Mauro Boselli bagged a brace as Leon routed 10-man Atlas 4-0 at the Estadio Leon.
The comprehensive victory saw Leon (21 points, +3) move into the eighth and final Apertura play-off spot with two rounds remaining after Cruz Azul (20 points, +3), Santos Laguna (19 points, -2), Queretaro (18 points, +0) and Pumas UNAM (18 points, +0) all dropped points.
Atlas (27 points, +1) slipped from second position to fourth after losing twice in three matches.
On a three-game winning streak, Leon went into the fixture full of confidence and it took the host just eight minutes to assert its dominance.
Boselli made no mistake from the penalty spot, sending Atlas goalkeeper Federico Vilar the wrong way.
Leon ran rampant in the second half, with Carlos Pena heading home from close range seven minutes after the break.
Boselli had his second goal seven minutes later and substitute Yamilson Rivera got in on the action in the 68th minute, albeit with some help from a deflection.
Atlas played the final 18 minutes with 10 men after midfielder Rodrigo Millar's red card.
Leon climbed into the play-off positions after Cruz Azul played out a goalless draw with sixth-placed Chiapas, Santos Laguna drew 1-1 at third-bottom Universidad Guadalajara and Queretaro lost 1-0 to Tigres UANL (27 points, +7), which jumped to third ahead of Atlas on goal difference.
Pumas UNAM drew for the third consecutive week, held to a scoreless stalemate by Veracruz.
League leader America dropped points for the second week in a row after drawing 0-0 against second-bottom Guadalajara in Mexico's biggest derby.
Neither side could find the back of the net in the 'El Super Clasico', despite a hectic finale at the Estadio Azteca.
America remains top of the standings with 30 points and a goal difference of +11, despite only collecting four points from a possible 12 on offer in its past four matches.
Toluca (28 points, +7) closed within two points of the pacesetter thanks to a hard-fought 1-0 win at Tijuana.
Isaac Brizuela's 25th-minute goal was all that separated the two teams, as Tijuana (17 points, +1) was dealt a blow to its play-off hopes.
In other results, cellar-dwelling Morelia stunned fifth-placed Monterrey 2-1 and Ariel Nahuelpan's penalty earned Pachuca - seventh in the table - a 1-1 draw at Puebla.