La Liga Fest raised more than €625,000 (A$1.1 million) on Sunday (AEDT) as Barcelona stalwart Gerard Pique spoke of the need for unity amid the coronavirus pandemic.
Where Saturday nights would normally consist of fixtures in Spain's top flight, the live action was restricted to performances on an internet stream.
A host of La Liga stars - including Pique - addressed supporters, while musicians performed at the online festival.
Fans were encouraged to donate to the Banco Santander Foundation, where the money would be used to buy medical equipment to combat COVID-19.
There have been over 72,000 confirmed cases of the virus in Spain, with close to 6,000 deaths.
The total raised ticked past €625,000 as the festival ended, although donations would continue to be welcomed until Sunday afternoon (Monday AEDT).
Barca's Pique was the most high-profile star to address those watching, as he claimed players and supporters alike could be "invincible together".
"Without training, at home and trying to stay in shape, it is not easy," the World Cup winner said. "We will get through it between all of us.
"It is a difficult situation, but doing something like what La Liga is doing makes us feel proud of the players and the fans.
"It is something unique that means any La Liga fan is able to contribute their grain of sand. I am very happy to be able to participate in such an initiative.
"There is rivalry when we are on the field of play, but when people need us, we are united.
"There will be a cost, it is difficult, we will have difficult times. Others will suffer it in person, but we have to make it through together. We are all invincible together."