Internacional knocked out

Penarol's Fernado Martinuccio (Getty)

Defending champions Internacional were knocked out of the Copa Libertadores with a surprise 2-1 defeat to Penarol in the second leg of their last-16 clash.

With the tie poised at 1-1 following the opening match, Inter took the lead early through Oscar's left-footed strike.

However, Fernando Martinuccio equalised for Penarol a minute after the interval before an Olivera header five minutes later sent the Uruguayan side through.

They will now take on Universidad Catolica in the quarter-finals, with the Chileans seeing off Gremio 1-0 to complete a 3-1 aggregate win.

Milovan Mirosevic's goal three minutes from time settled the contest in Santiago.

Universidad coach Juan Antonio Pizzi is keen to keep its good run going against Penarol.

"We have the ambition to keep progressing and do important things for the club," he told Fox Sports.

"The 16 teams in this round all had what it takes to win the trophy. Now we are down to eight and the level is high for the quarter-final stage."

Once Caldas claimed an unexpected 3-2 aggregate win over Cruzeiro thanks to a 2-0 second-leg success.

Diego Amaya headed home a corner to open the scoring in the 66th minute before Dayro Moreno doubled the lead five minutes later.

Both teams ended the game with 10 players as Cruzeiro midfielder Roger and Once Caldas forward Carlos Carbonero were shown red.

Santos will be the next opponents for Once Caldas.

Asuncion overturned a 3-1 first-leg deficit to knock Brazilian champions Fluminense out of the competition.

Rodrigo Rojas, Miguel Samudio and Jose-Ariel Nunez were all on the scoresheet as the Paraguayans claimed a 3-0 victory in the return match.

Fluminense also had defender Mariano sent off in stoppage time.