Ajax could have forward Derk Boerrigter and full-back Gregory van der Wiel back for the Eredivisie title run-in after both made comebacks from injury in the reserves.
Boerrigter has been sidelined since last November with a back injury but scored twice in the 4-1 win at Heracles.
"It's really good to be back on the pitch," Boerrigter told the club's official website, www.ajax.nl. "To score twice is nice as well."
"I was given painkillers beforehand but could play freely and I quickly found my rhythm."
Both players could now play a part as Ajax look to retain their Eredivisie title.
Ajax is one-point clear of rival AZ with six games remaining.