The Brazilian Football Federation (CBF) has urged FIFA to apply punitive measures upon Colombia right-back Juan Camilo Zuniga.
Neymar, scorer of four FIFA World Cup goals so far in the tournament, was forced from the field in the closing stages of Brazil's 2-1 quarter-final win after a clumsy challenge from the full-back.
Subsequent scans showed the Barcelona star had suffered a cracked vertebra, ruling him out for the remainder of the competition.
The incident went unpunished by referee Carlos Velasco, much to the chagrin of Brazilian fans who know their side faces a semi-final against Germany without the attacking focal point.
The CBF has thrown its weight behind an attempt to see Zuniga given a retrospective punishment for his part in Neymar's injury.
"The CBF announced that, because of what happened yesterday in the match played between the national teams of Brazil and Colombia, we have requested the FIFA Disciplinary Committee to open a disciplinary procedure to investigate and adjudicate the violent tackle of Colombian soccer player [Juan Zuniga] who hit [Neymar], keeping him out fo the World Cup," a CBF statement read.