Lewis Cook has suffered a ruptured anterior cruciate ligament and could be out for up to nine months, Premier League club Bournemouth have confirmed.
The 21-year-old midfielder sustained the injury during his side's 2-1 win over Huddersfield Town in midweek – a result that lifted the Cherries up to seventh in the table.
Cook won his maiden England cap during March's friendly against Italy before returning to the Under-21 set-up.
He will now miss the rest of the season, ruling him out of contention of England's opening EURO 2020 qualifiers and the inaugural UEFA Nations League Finals in June.
"AFC Bournemouth midfielder Lewis Cook will be sidelined for six-to-nine months with a ruptured anterior cruciate ligament," a club statement read.
"Cook was assessed by the club's medical staff following the match, with further assessment and scans on Wednesday revealing the extent of the damage to his right knee."
Bournemouth manager Eddie Howe said: "We are all devastated for Lewis. It is a huge blow to a very talented player who has been a very important part of our side over the last 18 months.
"But Lewis is a strong character who only has to look around our dressing room for inspiration of how an injury like this is merely a setback and to know that he can come back better than ever.
"He will have the full support and love of everyone at the club throughout the next few months as he receives the very best treatment and rehabilitation possible."
Bournemouth, who are back in action at home to title chasing Liverpool this weekend, have had to deal with a succession of knee injuries to first-team players since promotion to the Premier League in 2015.
Defender Adam Smith will be sidelined into the new year with a cartilage tear, while top scorer Callum Wilson has twice sustained similar setback to the one Cook must endure.
Tyrone Mings missed the vast majority of the 2015-2016 campaign after suffering torn knee ligaments 12 minutes into his debut against Leicester City.