MLS will resume play at Disney World Resort in Orlando on July 8, and a pair of Florida teams have the honour of kicking off the tournament with Orlando City facing David Beckham's newcomers Inter Miami.
A day after the MLS is Back Tournament was announced, the league held a draw for the group stage on Thursday, placing the 26 teams into six groups.
Sixteen teams will then progress to the knockout stage, which will begin on July 25 ahead of an August 11 final.
Aside from the opening match, the rest of the tournament schedule has yet to be announced.
The groups for the MLS is Back Tournament:
Group A (Eastern Conference): Orlando City, Inter Miami, New York City, Philadelphia Union, Chicago Fire, Nashville SC.
Group B (Western Conference): Seattle Sounders, Dallas, Vancouver Whitecaps, San Jose Earthquakes.
Group C (Eastern Conference): Toronto, New England Revolution, Montreal Impact, DC United.
Group D (Western Conference): Real Salt Lake, Sporting Kansas City, Colorado Rapids, Minnesota United.
Group E (Eastern Conference): Atlanta United, FC Cincinnati, New York Red Bulls, Columbus Crew.
Group F (Western Conference): Los Angeles FC, LA Galaxy, Houston Dynamo, Portland Timbers.