Seven of City's players have spent time at Adelaide United, who were Victory's most bitter rivals before the introduction of a second A-League team in Melbourne eight years ago.
The contingent includes former Reds skipper Eugene Galekovic, Stefan Mauk, Osama Malik, Scott Jamieson, Bruce Kamau and foreigners Marcelo Carrusca and Iacopo La Rocca.
Five of those players started in City's 2-0 win over Brisbane Roar last Friday and the other two, Carrusca and Jamieson, were on the bench, while director of football Michael Petrillo and assistant coach Michael Valkanis are also renowned for their time at United.
"Even when I was playing over in Adelaide... coming over here, it's a bit bigger," Kamau said. "It's a natural rivalry. It is always a big game.
"The derbies last year were quite good, especially towards the start of the season - there's a bit more of a hype around it. I think we're ready for it."
Mauk, meanwhile, told News Corp he had "grown up not liking Melbourne Victory" as a youngster in Adelaide and still felt that way.
The fringe Socceroo nearly wound up playing under Kevin Muscat this season as he sought a return to the A-League on loan from his Dutch club NEC.
Instead he returned to City, where he began his professional career in 2012.
"That rivalry has stayed from my end," Mauk said.
Kamau admitted his two-goal haul against the Roar disguised what he said was a sub-par performance, having notably fluffed his lines in attack a number of times in the first half.
"To be honest I don't think I played that well," he said.
"It's my personal opinion after watching the game back, we've got a computer in the club where we have our own individual clips... but (scoring) will obviously help lift that confidence."
City have won eight of the 23 Melbourne derbies to date, including an A-League fixture and an FFA Cup clash last season, but suffered losses in the most recent two clashes.
Victory will be at full strength with Mark Milligan, James Troisi and Kosta Barbarouses to return from international duty after missing the 1-0 defeat to Sydney FC at the weekend.