Football Federation Australia has received confirmation the clash will be held at the Hang Jebat Stadium on October 5 in Malacca, with the return match to be played on October 10 at Sydney's ANZ Stadium.
It follows a week of uncertainty after attempts by the Syrian football federation to have it staged in Qatar or the United Arab Emirates, with the Asian Football Confederation eventually intervening to lock in a venue.
Syria played its last match in Syria in 2010, before civil war hit the country and they have had to play their international fixtures elsewhere.
They were at a loss of where to host their matches for the World Cup qualifiers and were nearly disqualified by the AFC, until Malaysia stepped up at the eleventh hour and volunteered to host all of the 'home' fixtures.
With it being considered likely that Saudi Arabia would be the likely opponents in the result of a play-off, the more humid climate in Malaysia was considered likely to give the now aclimatised Syrians an advantage, but the fall of the Socceroos meant that they were instead looking for a neutral Middle East site instead.
Delays have meant that the AFC decided to step in and force the decision and confirm the Malaysian site.