Adelaide United coach Josep Gombau called Cassio's dressing room absence in the FFA Cup final "disrespectful" and refused to select the injured defender again.
Despite having several players injured and just a three-day turnaround for the game against Newcastle Jets at Hunter Stadium, Gombau said Cassio would not be selected on principle.
The coach was angered with the Brazilian's noticeable absence from the Adelaide dressing room before and after the side's FFA Cup win over Perth Glory on Tuesday.
"Cassio has something that I don't like and that is he didn't come before the game to say to the players `good luck' and after the game he didn't come to celebrate with the players," Gombau said.
"For me this is disrespectful. No chance."
Eyebrows were raised when Cassio was not presented with a squad medal after the FFA Cup triumph.
Asked if the left-back could be released by the Reds during the January transfer window, Gombau said that the decision was up to Adelaide management but did not rule it out.
Cassio has been on the long-term injury list with a hamstring problem.
He was named in the matchday squad against Western Sydney a fortnight ago but did not make it onto the pitch.
Gombau revealed that several Adelaide players had bumps and bruises in the aftermath of the FFA Cup triumph and the club would travel with an extended squad to Newcastle.
The coach confirmed Craig Goodwin would play against his former side for the first time.
The 23 year-old was forced to sit out the Reds' FFA Cup campaign after making the switch from Newcastle to South Australia in September.
He played in the Jets' FFA Cup round-of-32 game before departing, making him ineligible for the Reds' run to the trophy.
Newcastle striker Joel Griffiths said the struggling Jets were wary of Adelaide, who he described as the best team in the competition, and held no grudges against his former team-mate.
"They've got some players that are playing out of their skins this year, Craig Goodwin is one of them, which is good because he's a good guy," Griffiths said.
"We're up against it again. I honestly believe we can beat any team on any given day, I really do.
"But it just feels like some teams have that little X-factor."
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