The point from a goalless draw at FC Vestsjaelland proved enough to earn AaB its fourth Superliga title as nearest challenger FC Midtjylland slipped up on the road.
The leader knew clinching victory at Vestsjaelland would most likely see it crowned Danish champion for the first time since 2008, but it could just not get the ball over the line in Slagelse.
FC Midtjylland could therefore have closed to within a point of the leader ahead of the final round of fixtures by beating FC Copenhagen, but it instead lost 3-2 to concede defeat in the title race.
Morten Rasmussen opened the scoring from the penalty spot after 14 minutes but goals from Rurik Gislason (47) and Andreas Cornelius (53) saw FCK turn the game on its head.
After Rasmussen equalised in the 64th minute, Georg Margreitter was sent off for the capital club, which negotiated the dismissal well enough to produce a last-minute winner from Cristian Bolanos.
Copenhagen now leads Brondby by two points in the race for third after Thomas Frank's side was held to a 1-1 draw by Randers.
Ronnie Schwartz fired eighth-placed Randers ahead on the half hour before Simon Makienok Christoffersen levelled in the 63rd minute.
AGF Aarhus and Viborg were both relegated after losing their respective home games.
Basement boy Aarhus had Alexander Andersen sent off on the way to suffering a 3-0 defeat to Sonderjyske - Hallgrimur Jonasson, Tommy Bechmann and Rubin Okotie scored the goals.
Viborg's fate was sealed by FC Nordsjaelland, which ran out a 3-1 winner in Jutland thanks to first-half goals from Emiliano Marcondes, Jens Stryger Larsen and Joshua John.
Thomas Dalgaard responded for Viborg with a penalty before the hour but last year's Danish 1st Division champion must make an instant return to the second tier.
Elsewhere, Esbjerg consolidated itself in fifth place with a 2-1 victory at Odense.
Martin Pusic and Rasmus Falk Jensen exchanged goals in the first half before Jakob Ankersen hit the winner for Esbjerg 12 minutes from time.