Tottenham is still waiting to discover how long left-back Assou-Ekotto will face on the sidelines amid fears he could be ruled out for up to a month.
The 28-year-old missed
Losing Assou-Ekotto, who has barely missed a match in the last four seasons, would be seen as a blow to Tottenham's hopes of building on its opening Premier League victory this term.
Jan Vertonghen has filled in at left-back numerous times for
Despite playing out of position, the 23-year-old proved up to the challenge at the
"I played at left-back a few times for Norwich [on loan] last season and hopefully now I can carry on playing.
"Towards the end, I was tiring a little and they got in behind us a little but the more games I play, hopefully the stronger I'll get.
"Everyone's been brilliant and I just hope I can carry on now and play in the Europa League."
The Group J match is Tottenham's first ever competitive meeting with Lazio, to which it famously sold
The draw last month prompted the Italian giant to offer to invite the former England midfielder to the reverse fixture in
Press Association Sport understands the 45-year-old was also invited to attend this week's match but he was unable to accept for personal reasons.
Gascoigne has struggled to deal with alcohol problems since retirement but his agent said this northern summer that he had embarked on a health drive.
It remains to be seen whether he will attend the game between the sides at the Stadio Olimpico.
Meanwhile, Villas-Boas vowed to take the Europa League seriously ahead of his first match in the competition since winning it with Porto last year.
"We want to do something special in this competition," he said.
"This competition is very important.
"It's a tough competition. You have to play 15 games before you reach the final, so it's hard and strenuous and the best teams out of the Champions League are playing.
"It gives access to the European Super Cup and, for me, I always thought it was a prestigious competition and it should be promoted like that because after the Cup Winners' Cup disappeared, it is not only about the Champions League.
"If we can win a trophy, that represents a lot for Tottenham Hotspur's history as well and would be something really special."
Lazio has begun Serie A with a hat-trick of wins and Tottenham's northern summer signing
"It's great to play in
"It will be important to get three points at home."
UEFA Europa League
Malaga has been able to reduce its suspension from competing in European competitions to one year.