Australia will take on United States at The Home Depot Center in California on 17 September, Football Federation Australia (FFA) announced.
Arranged for the US team's post-Olympic return, the match will kick off at 5.30am (AEST).
The Matildas are looking for their first ever win against world number one-ranked United States, which has won 20 of the 22 matches played between the two sides.
Australia will be without Heather Garriock (pregnant) and the injured Ellise Kellond-Knight and Lydia Williams for the match, but will be able to call on United States-based stars Kyah Simon and Emily van Egmond.
The first match between Australia and USA resulted in a 6-0 win to the US on 16 December, 1987, in Taipei, Taiwan.A likely second match will be announced against the USA team in the coming days.
John Boultbee is set to relinquish his role as Football Federation Australia’s (FFA) Head of National Teams to take up a role as honorary head of World Rowing (FISA).