Australia is out for revenge against Japan when the two sides clash on Wednesday, having been knocked out of Olympic games qualifying by the world championi last September.
Matildas coach Tom Sermanni said the invitation to play Japan in its final Games tune-up in Tokyo on Wednesday is testament to the quality of the Australian side.
"When you play a team of the stature of Japan and how well they've done, particularly in the last year, it would be fantastic for us if we could actually get a victory and we will be trying to do that but we know it will be very difficult," Sermanni said.
"But for us to get an invitation to come here and play Japan in their final game before the Olympics is a real honour for us and for our players."
While the Matildas are coming off a successful Trans-Tasman series with New Zealand in which they posted a win and a draw, defender Danielle Brogan said Japan will be a much tougher assignment.
"New Zealand were a quality team but coming up against world champions Japan is going to be a very different game," Brogan said.
"I expect it to be even quicker, so we just have to make sure we fight it out for the full 90 minutes and not let them in.
"We just have to back ourselves. We're in the top 10 so we shouldn't underestimate our own ability.
"You want to pay Japan respect but not enough that you start digging your own grave."
Uncapped duo Emily Gielnik and Katrina Gorry will make their Matildas debut in the National Stadium match after stand out seasons in the W-League, while established stars Kyah Simon, Tameka Butt and Servet Uzunlar joined the squad from their foreign bases.
Matildas squad: Melissa Barbieri (gk, capt), Brianna Davey (gk), Laura Alleway, Danielle Brogan, Stephanie Catley, Thea Slatyer, Brooke Spence, Servet Uzunlar, Teigen Allen, Tameka Butt, Katrina Gorry, Alanna Kennedy, Ashley Brown, Caitlin Foord, Emily Gielnik, Kathryn Gill, Kyah Simon, Sarah Walsh.
John Boultbee is set to relinquish his role as Football Federation Australia’s (FFA) Head of National Teams to take up a role as honorary head of World Rowing (FISA).