Title-challenging Liverpool topped the table with £43.7 million ($80m) going to agents for the 12 months ending Jan 31 - more than Manchester City and Manchester United combined.
Liverpool invested in new players before the start of the current season, spending about £177 million ($325m) on the likes of Roma keeper Alisson, Naby Keita from Leipzig, Fabinho from Monaco and Xherdan Shaqiri from Stoke City.
Chelsea were the second-highest spenders on agents fees with £26.8 million ($50m) paid out, followed by Manchester City's £24 million ($44m) . Cardiff's £2.8 million ($5m) was lowest of the 20 clubs.
Tottenham Hotspur spent £11 million ($20m) on intermediaries despite making no signings in the last two transfer windows.
Second-tier (Championship) clubs spent a combined £50 million ($92m) on agents fees.
The Premier League's 20 shareholders are set to discuss new ways to reduce agents fees at a meeting on Friday - possibly ending the system whereby agents are paid by both the buying club and the player.
World governing body FIFA said last September it was considering proposals to reform the transfer system and restrict loans and agents' fees.
