Borussia Dortmund aim to hold off Hoffenheim in the race for third place and direct Champions League entry while, in the lowlands, third-last SV Hamburg face fourth-bottom Wolfsburg in a do-or-die match for instant survival.
Europa League spots are also up for grabs in the season-ending round.
Bayern, clinching a record fifth-straight title with three games to spare, will want to end the season on a high against sixth-placed Freiburg who are one of the Europa League contenders.
Lahm, 33, will play his final match as he ends a 14-year career with 21 titles for Munich and the 2014 World Cup. Spanish midfield veteran Xabi Alonso, winner of the Champions League with Liverpool and Real Madrid, is also calling it quits at age 35.
Lahm admitted he would probably have some jitters as he took to the pitch one last time.
But overall, he is "looking forward to the last game, to be with the team one last time, with the fans, with the staff in the stadium. I can't influence what else will happen".
Striker Robert Lewandowski wants to win the top scorers' crown for a second consecutive year but, with 30 goals, is just one ahead of Dortmund's Pierre-Emerick Aubameyang.
Dortmund and Hoffenheim are tied on points but Dortmund are ahead by four goals as they host Europa League contenders Werder Bremen in a game which could also determine the future of coach Thomas Tuchel.
Julian Nagelsmann's Hoffenheim entertain Augsburg who could plunge into the relegation playoff spot if they lose by two goals more than Wolfsburg in Hamburg.
Hamburg, the only founding league member in 1963, have never been relegated but are on the verge of a third relegation playoff tie in four seasons.
A last-minute 1-1 equaliser at Schalke spared them the direct drop but, trailing Wolfsburg, Augsburg and Mainz by two points, must win on Saturday to avoid another playoff.
The former Bundesliga and European champions plan a great escape against Wolfsburg who won the league in 2009 but only avoided relegation two years later on the last match day.
Fifth-placed Hertha Berlin host Bayer Leverkusen, Borussia Moenchengladbach meet relegated Darmstadt, Frankfurt play runners-up RB Leipzig and relegated Ingolstadt face Schalke.